The current study was performed to determine the frequency and drug resistance and biotypes of enterococcus-related urinary tract infections (UTI) in a center in Tehran. In this observational cross-sectional descriptive study, 2235 consecutive patients with suspected UTI were enrolled and evaluated for frequency, drug resistance, and biotypes of enterococcusrelated urinary tract infections. Our findings revealed that 2589 subjects (6.5%) had established UTI among which 87 subjects (3.4%) exhibited enterococcus-related urinary tract infection. Among them 70 cases were evaluated for biotype and drug resistance that all cases were Faecalis biotype. The nitrofurantoin (6%) and gentamicin (85.9%) were found to be the lowest and most drug resistance, respectively.,Ceftizoxime, ciprofloxacine, co-trimoxazole ampicillin, and nalidixic acid were among the least active agents for the UTI isolates of E. coli Morovere, a divers rang of antibiotic resistance has been shown for other antibiotics in.the present studyUTI isolates of klebsiella showed resistance to ampicillin, followed by
staphylococcus aureus (penicillin and tetracycline), staphylococcus epidermis isolates (penicillin), staphylococcus saprophyticus (gentamycin, penicillin, and cloxacillin), and streptococcus viridans ciprofloxacin (44%) and tetracycline (83%). Our findings revealed that E coli is the most common cause of urinary tract infection in both men and women. Enterococcus is responsible for three percent of urinary tract infections with dominant biotype of faecalis. The most sensitivity and resistance were related to nitrofurantoin and gentamicin, respectively.